Regulatory

Part:BBa_K113012:Design

Designed by: Wu Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-26)


weaken overlapping T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is difficult to make this short DNA by PCR as both sides are T7 promoters, which are very similar. It is highly possible that they form loops before they can be synthesized by PCR. Thus we order two single stranded DNA and then anneal them together by touchdown PCR.



Source

The sequence of T7 promoter is TAATACGACTCACTATA (from 5' to 3').


References